在这部福禄双霸天2000动作/喜剧/歌舞片中,18年后，当年因演唱会滋拢事件入狱的爱伍德终于出狱，但是昔日好友杰克已去逝，为了实现当年巡回的演唱梦想，他找到了一名十岁的孤儿巴斯特与酒保麦克，重组了蓝调兄弟乐团，打算南下路易西安那州，参加一场蓝调乐团的比赛。但是路途中，他们却被警方、莫斯科黑手党追赶，一路上惊险汽车追逐场面不断，最后蓝调兄弟合唱团是否能在这场蓝调音乐竞技会上一炮而红呢？
Elwood, the now lone "Blues Brother" finally released from prison, is once again enlisted by Sister Mary Stigmata in her latest crusade to raise funds for a children's hospital. Once again hitting the road to re-unite the band and win the big prize at the New Orleans Battle of the Bands, Elwood is pursued cross-country by the cops, led by Cabel the Curtis' son (and Elwood's step-brother), the Russian Mafia, and a militia group. On his new "mission from God" Elwood enlists the help of a young orphan, and a strip club bartender.
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